An approach to analysis of primary amines by a combination of thin-layer chromatography and matrix-assisted laser desorption ionization mass spectrometry in conjunction with post-chromatographic derivatization

On-spot derivatization has been suggested for the modification of primary amine containing compounds for their analysis by thin-layer chromatography hyphenated with matrix-assisted laser desorption ionization mass spectrometry. The proposed approach was based on post-chromatographic treatment of separated analytes inside the chromatographic zones on the thin-layer chromatography plates by tris(2,6-dimethoxyphenyl)methilium reagent. The derivatives, containing permanent positive charge, reveal exceptionally intense peaks of their cationic moieties and high signal/noise ratio in mass spectra recorded directly from the plates. The method was tested on a series of aliphatic, aromatic, and amine-containing pharmaceuticals. © 2019 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim

Авторы
Borisov R. 1, 2 , Esparza C. 2 , Polovkov N.1 , Topolyan A.3 , Zaikin V.1
Издательство
Wiley-VCH Verlag
Номер выпуска
22
Язык
Английский
Страницы
3470-3478
Статус
Опубликовано
Том
42
Год
2019
Организации
  • 1 A.V.Topchiev Institute of Petrochemical Synthesis, Russian Academy of Sciences, Moscow, Russian Federation
  • 2 People's Friendship University of Russia (RUDN University), Moscow, Russian Federation
  • 3 Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russian Federation
Ключевые слова
derivatization; mass spectrometry; matrix-assisted laser desorption/ionization; primary amines; thin-layer chromatography
Дата создания
24.12.2019
Дата изменения
24.12.2019
Постоянная ссылка
https://repository.rudn.ru/ru/records/article/record/54934/
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