Dependence of the sound-signal frequency on the animal body length was studied in 14 ground squirrel species (genus Spermophilus) of Eurasia. Regression analysis of the total sample yielded a low determination coefficient (R2 = 26%), because the total sample proved to be heterogeneous in terms of signal frequency within the dimension classes of animals. When the total sample was divided into two groups according to signal frequency, two statistically significant models (regression equations) were obtained in which signal frequency depended on the body size at high determination coefficients (R2 = 73 and 94% versus 26% for the total sample). Thus, the problem of correlation between animal body size and the frequency of their vocal signals does not have a unique solution. © 2017, Pleiades Publishing, Ltd.