Antitumor enzyme L-lyzine-α-oxidase from Trichoderma harzianum Rifai F-180 and investigation of its action on L-lysine oxidation by capillar electrophoresis

Trichoderma harzianum Rifai F-180, an organism producing the antitumor enzyme L-lysine-a-oxidase was cultivated and the enzyme was isolated and purified under the manufacturing conditions. The effect of L-lysine-α-oxidase on oxidation of L-lysine was investigated for the first time by capillary electrophoresis and the procedure conditions were developed. The reaction of L-lysine oxidative deamination is described and location of the reaction components picks on the elecrophoregrams was identified. The average rate of the catalytic reaction of L-lysine oxidation equal to 0.46 RU/min (7.7×103 RU/sec) was determined. The use of the antitumor enzyme L-lysine-a-oxidase is recommended as a drug for the treatment of superficial tumors and tissue relative oxygen excess.

Авторы
Smirnova I.P. 1 , Shkinev V.M.2 , Rudnev A.V.3 , Kuznetsova O.M. 1 , Karimova E.V. 1 , Orlova V.S. 1
Номер выпуска
3-4
Язык
Русский
Страницы
12-15
Статус
Опубликовано
Том
59
Год
2014
Организации
  • 1 Russian University of Peoples' Friendship, Moscow, Russian Federation
  • 2 D. I. Ivanovsky Research Institute of Virology, Moscow, Russian Federation
  • 3 V. I. Vernadsky Institute of Geochemistry and Analytical Chemistry, Moscow, Russian Federation
Ключевые слова
Antitumor enzyme; Capillary electrophoresis; L-lysine-α-oxidase; Trichoderma
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Varlamov A.V., Guranova N.I., Listratova A.V., Borisova T.N., Khrustalev V.N., Titov A.A., Voskressensky L.G.
Химия гетероциклических соединений. Латвийский институт органического синтеза Латвийской академии наук / Springer New York Consultants Bureau. Том 50. 2014. С. 264-270