Development and preclinical studies of orthotopic bone implants based on a hybrid construction from poly(3-hydroxybutyrate) and sodium alginate

The aim of the investigation was to develop a technology of manufacturing bone implants based on a hybrid polymer construction composed of poly(3-hydroxybutyrate) and sodium alginate for guided bone regeneration using 3D printing method. Materials and Methods. Complex shaped bone implants based on poly(3-hydroxybutyrate) and sodium alginate were manufactured by the method of two-stage leaching using a mold obtained by 3D printing. The appearance, morphology and structure of the obtained scaffolds were analyzed by means of scanning electron microscopy. Biocompatibility in vivo was determined based on the histology data of scaffolds implantation as bone substitutes. Results. The study of the developed hybrid 3D scaffolds from poly(3-hydroxybutyrate) and sodium alginate showed that they perform a restrictive function providing conditions for regeneration of flat cranial bones in rats. Conclusion. The developed hybrid 3D scaffolds do not interfere with normal osteogenesis and provide beneficial conditions for regeneration. © 2016, Nizhny Novgorod State Medical Academy. All rights reserved.

Авторы
Muraev A.A. 1, 2 , Bonartsev A.P.3, 4 , Gazhva Y.V.1 , Riabova V.M.1 , Volkov A.V. 5 , Zharkova I.I.3 , Stamboliev I.A. 2 , Kuznetsova E.S.6 , Zhuikov V.A.7, 8 , Myshkina V.L.4 , Mahina T.K.4 , Bonartseva G.A.4 , Yakovlev S.G.7 , Kudryashova K.S.3, 7 , Voinova V.V.1, 6 , Mironov A.A.10, 11 , Shaitan K.V.3 , Gazhva S.I.12 , Ivanov S.Y. 1, 2
Журнал
Sovremennye Tehnologii v Medicine
Издательство
Nizhny Novgorod State Medical Academy
Номер выпуска
4
Язык
Английский
Страницы
42-49
Статус
Опубликовано
Ссылка
Внешняя ссылка
DOI
10.17691/STM2016.8.4.06
Том
8
Год
2016
Организации
  • 1 Department of Maxillofacial Surgery and Implantology, Faculty of Doctors’ Advanced Training, Nizhny Novgorod State Medical Academy, 10/1 Minin and Pozharsky Square, Nizhny Novgorod, 603005, Russian Federation
  • 2 Department of Maxillofacial and Oral Surgery, Peoples’ Friendship University of Russia, 6 Miklukho-Maklay st, Moscow, 117198, Russian Federation
  • 3 Department of Bioengineering, Lomonosov Moscow State University, 1/12 Leninskie Gory, Moscow, 119991, Russian Federation
  • 4 Laboratory of Microorganism Nitrogen Fixation, Bach Institute of Biochemistry, Federal Research Centre “Fundamentals of Biotechnology”, Russian Academy of Sciences, Build. 2, 33 Leninskiy Prospekt, Moscow, 119071, Russian Federation
  • 5 City Clinical Hospital named after Ye.O. Mukhin, 17 Federativny Prospekt, Moscow, 111399, Russian Federation
  • 6 Lomonosov Moscow State University, 1/12 Leninskie Gory, Moscow, 119991, Russian Federation
  • 7 Institute of Biomedical Technologies, Nizhny Novgorod State Medical Academy, 10/1 Minin and Pozharsky Square, Nizhny Novgorod, 603005, Russian Federation
  • 8 Bach Institute of Biochemistry, Federal Research Centre “Fundamentals of Biotechnology”, Russian Academy of Sciences, Build. 2, 33 Leninskiy Prospekt, Moscow, 119071, Russian Federation
  • 9 Department of Biochemistry, Lomonosov Moscow State University, 1/12 Leninskie Gory, Moscow, 119991, Russian Federation
  • 10 Department of Neurotechnology, Institute of Biology and Biomedicine, Lobachevsky State University of Nizhni Novgorod, 23 Prospekt Gagarina, Nizhny Novgorod, 603950, Russian Federation
  • 11 Experimental Modeling Unit, Central Research Laboratory, Department of Normal Physiology named after N.Y. Belenkov, Nizhny Novgorod State Medical Academy, 10/1 Minin and Pozharsky Square, Nizhny Novgorod, 603005, Russian Federation
  • 12 Department of Dentistry, Faculty of Doctors’ Advanced Training, Nizhny Novgorod State Medical Academy, 10/1 Minin and Pozharsky Square, Nizhny Novgorod, 603005, Russian Federation
Ключевые слова
Biocompatibility; Bone implants; Bone tissue regeneration; Poly(3-hydroxybutyrate); Polyhydroxyalkanoates; Sodium alginate
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