HPLC-MS Method for Simultaneous Quantitative Determination of an Innovative Russian Gestagen and its Metabolites in Rat and Rabbit Blood Sera

An HPLC-MS method for simultaneous quantitative determination of a novel gestagenic pharmaceutical and two of its metabolites in rat and rabbit blood sera was developed and validated. The method was selective and specific. The detection limit for all analytes was 5 ng/mL; lower limit of quantitation, 10 ng/mL. Calibration curves for all analytes had the form y = ax + b. The correlation coefficients were >0.99. Matrix effects, degree of extraction, and stability of analytes in standard solutions, analytical samples, and the biomatrix with multiple freeze—thaw cycles were studied. © 2019, Springer Science+Business Media, LLC, part of Springer Nature.

Авторы
Stepanova E.S. 1 , Makarenkova L.M. 1 , Chistyakov V.V. 1 , Rybakov Y.L.2 , Gukasov V.M.2 , Fedotcheva T.A.3, 4 , Parshin V.A.3 , Votyakov V.A.3 , Shimanovskii N.L.3
Журнал
Pharmaceutical Chemistry Journal
Номер выпуска
12
Язык
Английский
Страницы
1016-1020
Статус
Опубликовано
Ссылка
Внешняя ссылка
DOI
10.1007/S11094-019-01944-X
Том
52
Год
2019
Организации
  • 1 People’s Friendship University of Russia (RUDN University), Moscow, 117198, Russian Federation
  • 2 Scientific Research Institute Federal Research Centre for Project Evaluation and Consulting Services, Moscow, 123995, Russian Federation
  • 3 N. I. Pirogov Russian National Research Medical University, Ministry of Health of the RF, Moscow, 117997, Russian Federation
  • 4 All-Russia Research Institute of Medicinal and Aromatic Plants (VILAR), Russian Academy of Agricultural Sciences, Moscow, 113628, Russian Federation
Ключевые слова
gestagens (progestins); gestobutanoyl; HPLC-MS; validation
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