Background: Conventional diamond bur preparation, air abrasion, and acid etching of hard dental tissues have become an integral part of modern restorative dentistry. However, their influence on the surface microstructure of anisotropic enamel is poorly elucidated. This study investigated the effect of acid etching on the micro-roughness of buccal and occlusal enamel surfaces after traditional treatment with a diamond bur and a subsequent air-abrasion with mixtures based on Al