Generalists and specialists decomposing labile and aromatic biochar compounds and sequestering carbon in soil

Biochar plays an important role in soil carbon (C) sequestration, while the effectiveness of sequestration is mostly determined by microbial preferences to utilize the different biochar components (i.e. labile substances or fused aromatic backbone). Thus, it is important to investigate the responses of the bacterial community and specific taxa, and associated C metabolic functions to biochar labile C (i.e. easily mineralizable C) or aromatic backbone (i.e. persistent C). Here, we separated the biochar components into labile C and aromatic backbone, and used the 16S sequencing in conjunction with the addition of an internal standard to quantify the absolute copy number of various bacterial taxa. The aromatic backbone decreased microbial metabolic quotient by 47 %, incorporating more C into biomass and contributing to C sequestration rather than releasing it into the atmosphere, while the labile C caused the opposite trends. The bacterial generalists utilizing aromatic backbone and labile C were mainly from Oxalobacteraceae (Proteobacteria) and Sporosarcina (Firmicutes). The bacterial specialists responsive for aromatic backbone were from Myxococcaceae and Flavisolibacter, while those responsive to labile C were from Ktedonobacteraceae and Bradyrhizobiaceae. Microbial abundance of specialists responsive to labile C was 3.3 times larger than those responsive to aromatic backbone, indicating the preferences of labile C specialists for energy sources and aromatic C specialists for slow utilization of persistent C in versatile habitats. Our work revealed microbial strategies for utilization of organic substances of very contrast availability, and their contributions to soil C sequestration depending on biochar components. © 2022 Elsevier B.V.

Авторы
Liu H. , Wang X. , Song X. , Leng P. , Li J. , Mazza Rodrigues J.L. , Hong Z. , Kuzyakov Y. , Xu J. , Dai Z.
Журнал
Издательство
Elsevier
Язык
Английский
Статус
Опубликовано
Номер
116176
Том
428
Год
2022
Организации
  • 1 Institute of Soil and Water Resources and Environmental Science, College of Environmental and Resource Sciences, Zhejiang University, 866 Yuhangtang Road, Hangzhou, 310058, China
  • 2 Shandong (Linyi) Institute of Modern Agriculture, Zhejiang University, Linyi, 276000, China
  • 3 Yiwu Seed and Plant Quarantine Station, Jinhua, China
  • 4 Linyi City Academy of Agricultural Sciences, Shandong, Linyi, 276012, China
  • 5 Department of Land, Air, and Water Resources, University of California, Davis, Davis, 95616, CA, United States
  • 6 Agricultural Experiment Station, Zhejiang University, Hangzhou, 310058, China
  • 7 Department of Agricultural Soil Science, University of Göttingen, Göttingen, 37077, Germany
  • 8 Peoples Friendship University of Russia (RUDN University), Moscow, 117198, Russian Federation
  • 9 The Rural Development Academy at Zhejiang University, Zhejiang University, Hangzhou, 310058, China
Ключевые слова
Absolute abundance; Carbon sequestration; Diversity; Generalist; Metabolic quotient; Specialist
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