The relevance of the article is due to the need to replace petroleum diesel fuel with fuels obtained from alternative raw materials. Rapeseed oil is considered as an alternative motor fuel. The properties of petroleum diesel fuel and rapeseed oil are analyzed. The influence of these properties of fuels on the characteristics of their injection and spraying is investigated. The deterioration of the quality of the injection and spraying processes was noted when the engine was switched from petroleum diesel fuel to rapeseed oil. When replacing diesel fuel with rapeseed oil, a slight increase in the maximum injection pressure and the duration of fuel supply was noted. Other parameters of fuel injection and atomization processes practically did not change. To achieve the required performance indicators of the diesel engine, it is necessary to implement measures that improve the quality of the workflow. These measures include the regulation of the fuel injection advance angle, optimization of the geometry of the flow part of the nozzle sprayer, two-phase fuel supply. It is advisable to switch from rapeseed oil to mixed biofuels and optimize their composition.