Microbial phosphorus recycling in soil by intra- and extracellular mechanisms

Abstract Rising global stoichiometric imbalance between increasing nitrogen (N) availability and depleting phosphorus (P) resources increases the importance of soil microbial P recycling. The contribution of extra- versus intracellular P (re-)cycling depending on ecosystem nutrient status is vastly unclear, making soil microorganisms a blind spot in our understanding of ecosystem responses to increasing P deficiency. We quantified P incorporation into microbial DNA and phospholipids by 33P labeling under contrasting conditions: low/high P soil × low/high carbon (C)NP application. By combining 33P and 14C labeling with tracing of microbial community biomarkers and functional genes, we disengaged the role of DNA and phospholipids in soil P cycling. Microorganisms in low P soil preferentially allocated P to phospholipids with an acceleration of phospholipids metabolism driven by C addition, which was strongly related to high abundances of microbial community members (e.g. some G-) with a fast phospholipids turnover. In high P soil, however, more P was allocated to DNA with a microbial functional shift towards DNA synthesis to support a replicative growth when sufficient C was supplied, which was coupled with a strong enrichment of fungal copiotrophs and microbial genes coding DNA primase. Consequently, adaptation to low P availability accelerated microbial intracellular P recycling through reutilization of the P stored in phospholipids. However, microorganisms under high P availability commonly adopted extracellular P recycling with release and reuse of DNA P by microbial death-growth dynamics. These results advance our understanding on microbial adaptation to P deficiency in soil by regulating component-specific P pathways and reflect the specific functions of phospholipids and DNA for P recycling.

Авторы
Chen Jie 1, 2, 3, 4 , Xu Han1 , Seven Jasmin3 , Zilla Thomas3 , Dippold M.A. 2, 5 , Kuzyakov Yakov 3, 4, 6
Журнал
Издательство
Oxford University Press (OUP)
Номер выпуска
1
Язык
Английский
Статус
Опубликовано
Том
3
Год
2023
Организации
  • 1 Research Institute of Tropical Forestry, Chinese Academy of Forestry, 510520 Guangzhou, China
  • 2 Biogeochemistry of Agroecosystems, Department of Crop Sciences, University of Göttingen, 37077 Göttingen, Germany
  • 3 Soil Science of Temperate Ecosystems, University of Göttingen, 37077 Göttingen, Germany
  • 4 Agricultural Soil Science, Department of Crop Science, University of Göttingen, 37077 Göttingen, Germany
  • 5 Geo-Biosphere Interactions, Department of Geosciences, University of Tuebingen, 72076 Tuebingen, Germany
  • 6 Peoples Friendship University of Russia (RUDN University), 117198 Moscow, Russia
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