The nature of the diet has a great influence on the microbial diversity of the gastrointestinal tract. The results of studying the actual nutrition of 25 students-basketball players by the frequency method are presented. The energy value of the diet was 3166±484 kcal/day, proteins accounted for 16% of the daily calorie content, fats - 44%, carbohydrates - 40%. A high consumption of saturated fatty acids was noted against the background of a low content of polyunsaturated fatty acids n-3 in the diet. The frequency of consumption of bakery products, potatoes, vegetables, fruits and fish was low, meat and meat products were in line with the recommended, and confectionery products were excessive. Optimization of the diet by reducing the amount of fats, increasing carbohydrates, including dietary fiber, will positively affect the composition of the intestinal microbiome, and the high species diversity of microflora, in turn, will contribute to the formation of adaptation to training and improve sports performance.