One of the most significant and common evolutionary events is rapid episodes of diversification, that is often resolved in phylogenetic poverty maybe because of the insistence on the widespread use of traditional genes (rDNA, mtDNA, and some nuclear protein-coding genes) that are unable to reveal resolutions for difficult phylogenetic problems. The Tachinidae are the second richest species of Diptera, giving a good case study for illustrating the effectiveness of different loci in revealing phylogenetic relationships. We try to clarify the activity of different genes that used and can be employed in the phylogenetic studies to interpret the rapid diversifications and understand relationships within Tachinidae.