The application of computerized optico-structural analysis (OSA) for the evaluation of morphofunctional peculiarities in cells and tissues

Criteria for the evaluation of morphofunctional peculiarities in the smooth muscle tissue of the myometrium and in human leukocytes were evolved by the author and his associates. They were obtained on a computer-controlled PROTVA scanning microscope-photometer by the optico-structural analysis method elaborated by Bogdanov (1966). Comparative studies of RNA, protein and SH- groups in the myometrium were conducted during pregnancy and in the presence of both normal and weak myometrical contractility during labor. The second and third factors in the scannograms [D(X) and S(K)] were shown to be highly informative for evaluation of the tissue system as a whole. Image processing of Feulgen- and Romanovsky-stained fetal, neonatal and adult human leukocytes was also carried out on a PROTVA microscope-analyser. Peculiarities were observed in the morphological and cytochemical features of neutrophils, eosinophils and small, medium and large lymphocytes. Fine differences were also found among small lymphocytes.