Фенольные соединения листьев лопуха

Проведено химическое изучение фенольных компонентов листьев лопуха и сопоставление их с составом сока листьев лопуха. Выделены гликозиды кверцетина и кемпферола, эфиры кофейной кислоты: рутин, кемпферол-3-рутинозид, метиловый эфир хлорогеновой кислоты, 1,5-дикофеоилхинная кислота.

Phenolic components of arctium lappa L. leaves

The investigation of phenolic compounds of the leaves Arctium lappa was carried out. The leaves of Arctium Lappa was extracted with 70% EtOH. The extract was evaporated up to aqueous solution with the following extraction with CHCl ₃, EtOAc and n-BuOH. The precipitate from the n-BuOH layer was twice chromatographed on the SG column, using CHCl ₃-MeOH as eluent in gradient regime and CHCl ₃-MeOH-H ₂O 61:32:6 in isocratic regime. There were isolated rutine and camppherole-3-rutinoside. The evapd n-BuOH layer was chromatographed on the same sorbent using as eluent petrol-EtoAc in gradient regime (up to 80 % EtOAc), EtOAc, EtOAc-MeOH (up to 12 % MeOH). The fraction, containing three phenolic compounds was rechromatographed on the same sorbent using CHCl ₃-MeOH as eluent in gradient regime (up to 9 % MeOH). There were isolated clorogenic acid methyl ester and 1,5-dicaffeoylqinic acid. The identification of the compounds was carried out by the ¹H-NMR, UV spectral data. The aqueous layer of the leaves contain the caffeic acid derivatives, which absent in the aqueous layer juice.