Molecular mapping of tumor heterogeneity on clinical tissue specimens with multiplexed quantum dots

Tumor heterogeneity is one of the most important and challenging problems not only in studying the mechanisms of cancer development but also in developing therapeutics to eradicate cancer cells. Here we report the use of multiplexed quantum dots (QDs) and wavelength-resolved spectral imaging for molecular mapping of tumor heterogeneity on human prostate cancer tissue specimens. By using a panel of just four protein biomarkers (E-cadherin, high-molecular-weight cytokeratin, p63, and α-methylacyl CoA racemase), we show that structurally distinct prostate glands and single cancer cells can be detected and characterized within the complex microenvironments of radical prostatectomy and needle biopsy tissue specimens. The results reveal extensive tumor heterogeneity at the molecular, cellular, and architectural levels, allowing direct visualization of human prostate glands undergoing structural transitions from a double layer of basal and luminal cells to a single layer of malignant cells. For clinical diagnostic applications, multiplexed QD mapping provides correlated molecular and morphological information that is not available from traditional tissue staining and molecular profiling methods. © 2010 American Chemical Society.

Авторы
Liu J.1 , Liu T.1 , Nie S.1 , Lau S.K.2 , Varma V.A.2 , Petros J.A.2, 5, 6 , Osunkoya A.O.2, 5, 6, 4 , Moffitt R.A. 3 , Caldwell M. 3 , Wang M.D. 3 , Young A.N.4 , Krogstad T.4 , Leyland-Jones B.6
Журнал
Издательство
American Chemical Society
Номер выпуска
5
Язык
Английский
Страницы
2755-2765
Статус
Опубликовано
Том
4
Год
2010
Организации
  • 1 Departments of Biomedical Engineering and Chemistry|Emory University|Georgia Institute of Technology
  • 2 Atlanta Veteran Affairs Medical Center (VAMC)
  • 3 Department of Urology
  • 4 Winship Cancer Institute|Emory University|School of Medicine
  • 5 Department of Pathology|Laboratory Medicine
  • 6 Departments of Biomedical Engineering and Electrical and Computer Engineering|Georgia Institute of Technology
Ключевые слова
biomarker; immunohistochemistry; multiplexing; prostate cancer; quantum dot; spectral imaging; tumor heterogeneity
Дата создания
08.07.2024
Дата изменения
08.07.2024
Постоянная ссылка
https://repository.rudn.ru/ru/records/article/record/123324/
Поделиться

Другие записи

Воротникова С.Ю., Зотов А.К., Зотова Т.Ю., Тарасова Е.С.
Вестник новых медицинских технологий. Федеральное государственное бюджетное образовательное учреждение высшего образования Тульский государственный университет. Том 17. 2010. С. 210-212