Регуляция апоптоза нейтрофилов как параметра патологии различного генеза

В представленной работе исследовали эффекты стрессорнных гормонов, блокаторов адренергических рецепторов, метаболических модуляторов (используя как тест-систему влияние γ-радиации и УФС-ультрафиолетовое излучение с длиной волны 254 нм) на апоптоз нейтрофилов человека in vitro.

Regulation of polymorphonuclear leukocyte apoptosis as parameter of the pathology of different genesis

It was found, that UVC (254nm) in dose range 6-600 J/m² in vitro accelerates apoptosis of human peripheral blood neutrophils in dosedependent manner, with the saturation occurs under UVC doses of 250-300J/m². γ-irradiation in dose of 2Gr asselerates neutrophils apoptosis, whils the irradiation in doses of 10 and 20 Gr suppress it (for the 9 hours of the cultivation). LPS (lug/ml) suppress UVC-induced neutrophils apoptosis. At use of glucocorticoids observed reduction of expressiveness apoptosis process. Antiadrenergic drug operated in the opposite direction; the percent(interest) apoptosis PMN in the given technique of research has been increased by 15%. Blockade (antiadrenergic drug) restored (slowed down) expressiveness apoptosis process. These data show, that sensitivity polymorphonuclear cells to apoptosis to stimulus differs from sensitivity immune system.