Testing a Hypothesis of 12S rRNA Methylation by Putative METTL17 Methyltransferase

Mitochondrial ribosome assembly is a complex multi-step process involving many additional factors. Ribosome formation differs in various groups of organisms. However, there are universal steps of assembly and conservative factors that have been retained in evolutionarily distant taxa. METTL17, the object of the current study, is one of these conservative factors involved in mitochondrial ribosome assembly. It is present in both bacteria and the mitochondria of eukaryotes, in particular mice and humans. In this study, we tested a hypothesis of putative METTL17 methyltransferase activity. MALDI-TOF mass spectrometry was used to evaluate the methylation of a putative METTL17 target - a 12S rRNA region interacting with METTL17 during mitochondrial ribosome assembly. The investigation of METTL17 and other mitochondrial ribosome assembly factors is of both fundamental and practical significance, because defects in mitochondrial ribosome assembly are often associated with human mitochondrial diseases.

Авторы
Mashkovskaia A.V.1 , Mariasina S.S. 1, 2, 3 , Serebryakova M.V. 4 , Rubtsova M.P.1 , Dontsova O.A.1, 4, 5, 6 , Sergiev P.V.1, 2, 4, 5
Издательство
Общество с ограниченной ответственностью «АКТА НАТУРЭ»
Номер выпуска
4
Язык
Английский
Страницы
75-82
Статус
Опубликовано
Том
15
Год
2023
Организации
  • 1 Lomonosov Moscow State University
  • 2 Institute of functional genomics, Lomonosov Moscow State University
  • 3 RUDN University
  • 4 Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University
  • 5 Center for Molecular and Cellular Biology, Skolkovo Institute of Science and Technology
  • 6 Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
Ключевые слова
Mitochondrial ribosome; ribosome assembly factors; methyltransferases; RNA methylation; MALDI-TOF mass spectrometry
Дата создания
01.07.2024
Дата изменения
01.07.2024
Постоянная ссылка
https://repository.rudn.ru/ru/records/article/record/108342/
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Другие записи

Кобалава Ж.Д., Писарюк А.С., Филькова А.А., Тухсанбоев Ё.С., Болдырева А.А., Лазутова Д.П., Мерай И.А., Пантелеев М.А.
Rational Pharmacotherapy in Cardiology. Stolichnaya Izdatelskaya Kompaniya. Том 19. 2023. С. 614-627