Assessment of Proliferation and Apoptosis in Testes of Rats after Experimental Localized Electron Irradiation

Background and purpose: With the emergence of linear accelerators in radiotherapy, it becomes necessary to accurately select new dosing regimens. The purpose of this study was to assess the morphological changes of spermatogenesis after radiation exposure. Materials and methods: Male Wistar rats (n = 40) were subjected to targeted ionizing radiation on a pulsed electron accelerator “NOVAC-11” with doses of 2, 8 and 12 Gy. Spermatogenesis was assessed a week later using light microscopy and immunohistochemical method (antibodies to Ki-67, Bcl-2, p53, Caspase 3). Results: A decrease in the number of normal germ cells was seen in all experimental groups, while radioresistant Sertoli and Leydig cells were barely affected. The most serious damage to the tubules and germ cells was observed in 8 and 12 Gy irradiation groups. IHC analysis of testes after irradiation showed a shift in the proliferative-apoptotic balance toward apoptosis of germ cells: a decrease in the expression levels of Ki-67 and Bcl-2, an increase in p53-positive and caspase 3-positive cells by the end of the experiment. Conclusion: Dose-dependent progressive pathomorphological changes in histoarchitectonics of the testes are traced, and a decrease in the number of germ cells is seen on the seventh day after irradiation with a pulsed electron accelerator “NOVAC-11”.

Авторы
Demyashkin Grigory 1 , Koryakin Sergey2 , Moiseev Aleksandr2 , Saburov Vyatcheslav2 , Zatsepina Margarita3 , Epifanova Maya 1, 4 , Stepanova Yulia3 , Shchekin Vladimir3 , Vadyukhin Matvey3 , Shegay Petr 1 , Kaprin Andrei 1
Издательство
MDPI
Номер выпуска
11
Язык
Английский
Страницы
5768-5777
Статус
Опубликовано
Том
44
Год
2022
Организации
  • 1 National Medical Research Radiological Centre of the Ministry of Health of the Russian Federation
  • 2 A. Tsyb Medical Radiological Research Centre - branch of the National Medical Research Radiological Centre
  • 3 Institute of Clinical Medicine, I. M. Sechenov First Moscow State Medical University (Sechenov University)
  • 4 Department of Urology and Operative Nephrology, Peoples' Friendship University of Russia (RUDN University)
Ключевые слова
electron irradiation; male infertility; spermatogenesis; seminal balls
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