Evaluation of In Vitro Cytotoxic Potential of Avarol towards Human Cancer Cell Lines and In Vivo Antitumor Activity in Solid Tumor Models

The goal of this study was to determine the activity in vitro and in vivo of avarol, a sesquiterpene hydroquinone originating from the Dysidea avara sponge from the south Adriatic Sea, against different cancer cell lines and two types of mouse carcinoma. To investigate the in vitro cytotoxicity, a human cervix adenocarcinoma cell line (HeLa), human colon adenocarcinoma (LS174), human non-small-cell lung carcinoma (A549), and a normal human fetal lung fibroblast cell line (MRC-5) were used. The in vivo antitumor activity was investigated against two transplantable mouse tumors, the Ehrlich carcinoma (EC) and cervical cancer (CC-5). The effect of avarol on cancer cell survival, which was determined by the microculture tetrazolium test, confirmed a significant in vitro potency of avarol against the investigated cell lines, without selectivity towards MRC-5. The highest cytotoxicity was exhibited against HeLa cancer cells (10.22 ± 0.28 μg/mL). Moreover, potent antitumor activity against two tumor models was determined, as the intraperitoneal administration of avarol at a dose of 50 mg/kg resulted in a significant inhibition of tumor growth in mice. After three administrations of avarol, a 29% inhibition of the EC growth was achieved, while in the case of CC-5, a 36% inhibition of the tumor growth was achieved after the second administration of avarol. Therefore, the results indicate that this marine sesquiterpenoid hydroquinone could be a promising bioactive compound in the development of new anticancer medicine.

Авторы
Stanojkovic T.P.1 , Filimonova Marina2 , Grozdanic Nadja1 , Petovic Slavica3 , Shitova Anna2 , Soldatova Olga2 , Filimonov Alexander2 , Vladic Jelena4 , Shegay Petr 5 , Kaprin Andrey 5, 6 , Ivanov Sergey 2 , Nikitovic Marina1, 7
Журнал
Издательство
MDPI AG
Номер выпуска
24
Язык
Английский
Страницы
9048
Статус
Опубликовано
Том
27
Год
2022
Организации
  • 1 Department of Experimental Oncology, Institute of Oncology and Radiology of Serbia
  • 2 A. Tsyb Medical Radiological Research Center, Federal State Budget Institution National Medical Research Radiological Center of the Ministry of Healthcare of the Russian Federation
  • 3 Institute of Marine Biology, University of Montenegro
  • 4 Faculty of Technology, University of Novi Sad
  • 5 National Medical Research Radiological Center of the Ministry of Health of the Russian Federation
  • 6 Peoples' Friendship University of Russia, Medical Institute (RUDN University)
  • 7 Faculty of Medicine, University of Belgrade
Ключевые слова
avarol; Dysidea avara; Ehrlich carcinoma; cervical cancer; cytotoxicity
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