Objective of the present study was to assess the psychophysiological characteristics of students with different levels of motor activity and identify changes in the most important health indicators. Methods and structure of the study. The study involved the 16-18 year-old students with different motor activity modes. The following psychophysiologi-cal characteristics were assessed: vital capacity measured in liters by an air spirometer; heart rate, arterial blood and pulse pressure measured at rest using a semi-automatic OMRONM1 Compact tonometer; simple and complex visual-motor reaction, simple auditory-motor reaction; the tapping test was run using the hardware-software complex "Sports Psychophysiologist". All measurements were taken in view of the circadian and circaseptan biorhythms. The outcome test data were processed using the variation statistics method in accordance with the principles set out in V.B. Korenberg’s manual (2008). Results of the study and conclusions. The findings showed that additional physical loads have a positive effect on most parameters reflecting the activity of the main functional systems of the student’s body. Moreover, the greatest differences were observed in the vital capacity and sensorimotor response rates. © 2020, Teoriya i praktika fizicheskoy kul'tury i sporta. All rights reserved.