The article discusses the possibility of using various oilseeds for the production of biodiesel in the Samara region. Purpose of the work: to determine the economic efficiency of using various crops as the basis for the production of biodiesel, as well as to compare the options for the cultivation of spring rapeseed as the main crop for the production of biofuel. Within the framework of this, the following tasks were completed: a list of oilseeds was determined, the use of which is possible for the production of biodiesel in the Samara region conditions; analysis of the economic parameters of their cultivation; calculated the economic efficiency of various technologies for growing spring rape (6 options). It was revealed that crops such as spring rape, mustard and soybeans have the best economic parameters (cost, yield) in the Samara region. Among the technologies for the cultivation of spring rapeseed, the best results were shown by the following options: direct sowing with fertilizers and plowing without fertilizers (profitability 42.34% and 41.72%, respectively). © 2022, The Author(s), under exclusive license to Springer Nature Switzerland AG.