Assessing the biological effects of microwave irradiation on human semen in vitro and determining the role of seminal plasma polyamines in this process

The aim of the present study was to investigate the influence of millimeter-wave electromagnetic (MW) irradiation on normal and pathological human sperm in vitro, and to evaluate a possible role of polyamines (PA) in this process. The stability of sperm membranes, the number of apop-totic gametes, and the content of seminal plasma PA in the ejaculates of fertile and subfertile men were compared before and after short-term MW electromagnetic exposure in vitro. The ejaculate samples were collected from healthy donors [n=25, age 22-38 years old (y.o.), average age 30.6±1.1 y.o. (mean ± SEM)] and from subfertile men (n=78, age 25-48 y.o., average age 34.1±0.8 y.o.) and exposed to MW radiation. The electromagnetic field had a wavelength of 7.1 mm, a frequency of 42.194 GHz and an exposure time of 20 min. The fragility of sperm membranes was evaluated by their resistance to sodium chloride solution (Milovanov test) and to acetic acid (Joel test). Acrosin activity was assayed spectrophotometri-cally. Apoptosis was determined by the externalization of phosphatidylserine on the outer side of the sperm membrane and propidium iodide staining. The PA levels were determined by agar gel electrophoretic fractionation. An increase in the resistance of sperm membranes, a decrease in acrosin activity, a decrease in the number of apoptotic gametes and a decrease in the seminal plasma PA concentrations were found after exposure of the native human sperm to low-intensity MW irra-diation. Two types of reactions were revealed for the subfertile samples. The results revealed positive bio-effects of specific microwaves on the human semen and the participation of PA in the realization of these effects. © 2022, Spandidos Publications. All rights reserved.

Authors
Ploskonos M.V.1 , Zulbalaeva D.F.2 , Kurbangalieva N.R.2 , Ripp S.V.2 , Neborak E.V. 3 , Blagonravov M.L. 4 , Syatkin S.P. 4 , Sungrapova K. 3 , Hilal A. 4
Publisher
Spandidos Publications
Number of issue
5
Language
English
Status
Published
Number
38
Volume
16
Year
2022
Organizations
  • 1 Astrakhan State Medical University of The Healthcare Ministry of Russian Federation, Astrakhan, 414000, Russian Federation
  • 2 Astrakhan Region State Budget-Financed Healthcare Institution ‘Family Healthcare and Reproduction Center’, Astrakhan, 414040, Russian Federation
  • 3 Department of Biochemistry, Peoples' Friendship University of Russia (RUDN University), Moscow, 117198, Russian Federation
  • 4 Department of Pathophysiology, Peoples' Friendship University of Russia (RUDN University), Moscow, 117198, Russian Federation
Keywords
apoptosis; millimeter-wave electromagnetic radiation; polyamines; seminal plasma; sperm
Date of creation
06.07.2022
Date of change
06.07.2022
Short link
https://repository.rudn.ru/en/records/article/record/83650/
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