Analysis of Expression of Cancer-Testicular Antigens on the Tumor Cell Cultures of Bladder Cancer

Non-specific methods of immunotherapy have been successfully used for more than 40 years for activation of immune system in case of treatment of urothelial carcinoma. Development of method of specific immunotherapy in case of bladder cancer (BC) is a current problem. Cancer-testis antigens (CTA) are the most promising target in the context of creation of antitumor vaccines because they are distinguished by marked immunogenicity, they are detected in different types of tumors and have limited expression patterns in healthy tissues of grown-up organism. Development of personified dendritic cell antitumor vaccines against BC is related not only to selection of optimal antigens for activation of dendritic cells, but also to determination of optimal parameters of cultivation of cells for provision of maximum CTA expression by tumor cells. The work presents data in relation to research of peculiarities of CTA expression by tumor cultures of BC in case of long-term cultivation.