Combined and singular effects of ethanolic extract of persian shallot (Allium hirtifolium boiss) and synbiotic biomin® IMBO on growth performance, serum-and mucus-immune parameters and antioxidant defense in zebrafish (danio rerio)

This study was carried out to evaluate combined and singular effects of ethanolic extract of Persian shallot (Allium hirtifolium Boiss) and synbiotic Biomin® IMBO on growth performance, innate immune responses, and antioxidant defense in zebrafish (Danio rerio). Fish with initial weight of 151.90 ± 0.31 mg were allocated in 21 10-L glass aquariums. The experimental groups were as follows: T1, control (without any supplementation); T2, 1% synbiotic; T3, 3% synbiotic; T4, 1% Persian shallot (as a medical plant); T5, 3% Persian shallot; T6, 1% Persian shallot and 1% synbiotic; T7, 3% Persian shallot and 3% synbiotic. At the end of the experiment (60 days), all treatments significantly showed higher final weight (FW), weight gain (WG), WG (%), and specific growth rate (SGR) compared with the fish fed on control diet. Furthermore, both synbiotic Biomin® IMBO and Persian shallot significantly improved intestine immune parameters including lysozyme, alternative complement hemolytic activity (ACH50), total immunoglobulin (total Ig), and myeloperoxidase (MPO) of ze-brafish compared to fish fed on control diet (p < 0.05). Also, in all experimental groups, hepatic catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx), and glutathione reduc-tase (GR) activities significantly increased compared to the control group. Whereas, the highest MDA level was observed in the control group compared to the treatments (p < 0.05). Moreover, skin mucus immune parameters of zebrafish have been noticeably improved with synbiotic Biomin® IMBO and Persian shallot compared to fish fed on the control diet (p < 0.05). The results indicate that synbiotic or Persian shallot supplemented diet could enhance the general health status of the zebrafish. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.

Ghafarifarsani H.1 , Hoseinifar S.H.2 , Talebi M.3 , Yousefi M. 4 , Van Doan H. , Rufchaei R.7 , Paolucci M.8
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  • 1 Department of Fisheries, Faculty of Natural Resources, Urmia University, Urmia, 5756151818, Iran
  • 2 Department of Fisheries, Faculty of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, 4918943464, Iran
  • 3 Fishery Group, Department of Natural Resources, Islamic Azad University (Lahijan Branch), Lahijan, 1477893855, Iran
  • 4 Department of Veterinary Medicine, Peoples’ Friendship University of Russia (RUDN University), 6 Miklukho-Maklaya St., Moscow, 117198, Russian Federation
  • 5 Department of Animal and Aquatic Sciences, Faculty of Agriculture, Chiang Mai University, Chiang Mai, 50200, Thailand
  • 6 Science and Technology Research Institute, Chiang Mai University, 239 Huay Keaw Rd., Chiang Mai, 50200, Thailand
  • 7 Inland Water Aquaculture Research Centre, Iranian Fisheries Science Research Institute (IFSIR), Agricultural Research Education and Extension Organization (ARREO), Bandar-e Anzali, 4314166976, Iran
  • 8 Department of Sciences and Technologies, University of Sannio, Benevento, 82100, Italy
Growth; Herbal medicine; Immunity; Synbiotic; Zebrafish
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