Paleoenvironmental reconstructions are commonly based on isotopic signatures of a variety of carbonate types, including rhizoliths and land-snail shells, present in paleosol-loess sequences. However, various carbonate types are formed through distinct biotic and abiotic processes over various periods, and therefore may record diverging environmental information in the same sedimentological layer. Here, we investigate the effects of carbonate type on delta C-13, delta O-18, and clumped isotope-derived paleotemperature [T(Delta(47))] from the Quaternary Nussloch paleosol-loess sequence (Rhine Valley, SW Germany). delta C-13, delta O-18, and T(Delta(47)) values of co-occurring rhizoliths (-8.2 parts per thousand to -5.8 parts per thousand, -6.1 parts per thousand to -5.9 parts per thousand, 12-32 degrees C, respectively), loess dolls (-7.0 parts per thousand, -5.6 parts per thousand, 23 degrees C), land-snail shells (-8.1 parts per thousand to -3.2 parts per thousand, -4.0 parts per thousand to -2.2 parts per thousand, 12-38 degrees C), earthworm biospheroliths (-11 parts per thousand, -4.7 parts per thousand, 8 degrees C), and "bulk" carbonates (-1.9 parts per thousand to -0.5 parts per thousand, -5.6 parts per thousand to -5.3 parts per thousand, 78-120 degrees C) from three sediment layers depend systematically on the carbonate type, admixture from geogenic carbonate, and the duration of formation periods. Based on these findings, we provide a comprehensive summary for the application of the three isotopic proxies of delta C-13, delta O-18, and Delta(47) in biogenic and pedogenic carbonates present in the same sediment layer to reconstruct paleoenvironments (e.g., local vegetation, evaporative conditions, and temperature). We conclude that bulk carbonates in Nussloch loess should be excluded from paleoenvironmental reconstructions. Instead, pedogenic and biogenic carbonates should be used to provide context for interpreting the isotopic signature for detailed site- and time-specific paleoenvironmental information.