Purple nut sedge (Cyperus rotundus L.) is a problematic weed in plasticulture tomato (Solanum lycopersicum L.) production in Iran as it is difficult to control due to its ability to penetrate plastic mulch. Field trials were carried out at the experimental area of the state farm Safadasht, Shahriar region, Iran, over the fall of 2015 and spring of 2016 to investigate PRE-and POST herbicide programs (PRE transplanting, POST transplanting, and combination of PRE-and POST transplanting) to control purple nut sedge in plasticulture tomato production. PRE herbicide treatment metribuzin and (S)-metolachlor were not effective when applied alone, and did not reduce purple nut sedge plants compared to the weedy control. POST transplanting application of halosulfuron did not significantly affect purple nut sedge plants at 12 wk after application (WAA) in the fall 2015, but treatment favorably reduced density of purple nut sedge at 16 WAA in both seasons. PRE transplanting application of metribuzin or (S)-metolachlor plus POST halosulfuron exerted the greatest control, and purple nut sedge numbers were eventually diminished in both seasons. Experimental treatments did not negatively affect tomato height and yields. Based on our findings, multiple PRE and POST herbicide programs were effective in greater purple nut sedge suppression compared to the lone application of PRE and POST herbicides. The herbicides were selected due to routine use by tomato producers in the area. Using active ingredients from the various herbicide families with different modes of action could facilitate effective management of herbicide-resistant purple nut sedge in tomato fields. © 2021, Instituto de Investigaciones Agropecuarias, INIA. All rights reserved.