Tranesteification of Jatropha curcus L. fixed oil gave biodiesel which is having of some heavy metals, such as, Nickel, Copper, Vanadium, Arsenic and Lead with high concentration that may be released during the combustion. Thus producing significant toxic impact on the engine and environment. Pulverized date stone adsorbent efficiently detoxify the crude biodiesel. The adsorption efficiency of metals has been determined by comparing the concentration of metals in the crude biodiesel before and after the passing through an adsorbent column.The effect of variation of adsorbent dose on adsorption efficiency and percentage of adsorption has been studied. The concentration of heavy metals in the biodiesel before treatment has been found to be 5.152, 1.283, 0.291, 1.137, 2.626, and 2.428 ppm for Fe, Ni, Cu, V, As and Pb respectively. Concentrations have revealed decrease after the treatment to 0.962, 0.222, 0.0327, 0.150, 0.314, and 0.021 ppm respectively. Pulverized date stone has shown marked efficiency in removing toxic heavy metals from Jatropha biodiesel. Adjustment of DS weight and length of column resulted in higher percent of metal adsorption. This technique is a contribution in the betterment of fuel via an eco-friendly method.