Magnetic levitational bioassembly of 3D tissue construct in space

Magnetic levitational bioassembly of three-dimensional (3D) tissue constructs represents a rapidly emerging scaffold- and label-free approach and alternative conceptual advance in tissue engineering. The magnetic bioassembler has been designed, developed, and certified for life space research. To the best of our knowledge, 3D tissue constructs have been biofabricated for the first time in space under microgravity from tissue spheroids consisting of human chondrocytes. Bioassembly and sequential tissue spheroid fusion presented a good agreement with developed predictive mathematical models and computer simulations. Tissue constructs demonstrated good viability and advanced stages of tissue spheroid fusion process. Thus, our data strongly suggest that scaffold-free formative biofabrication using magnetic fields is a feasible alternative to traditional scaffold-based approaches, hinting a new perspective avenue of research that could significantly advance tissue engineering. Magnetic levitational bioassembly in space can also advance space life science and space regenerative medicine. Copyright © 2020 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution License 4.0 (CC BY).

Parfenov V.A.1, 2 , Khesuani Y.D.1 , Petrov S.V.1 , Karalkin P.A.1, 3 , Koudan E.V.1 , Nezhurina E.K.3 , Pereira F.D.A.S.1 , Krokhmal A.A.1 , Gryadunova A.A.1 , Bulanova E.A.1 , Vakhrushev I.V.1 , Babichenko I.I. 4 , Kasyanov V.5 , Petrov O.F.6 , Vasiliev M.M.6 , Brakke K.7 , Belousov S.I.8 , Grigoriev T.E.8 , Osidak E.O.9 , Rossiyskaya E.I.10 , Buravkova L.B.11 , Kononenko O.D.12 , Demirci U.13 , Mironov V.A.1, 14
American Association for the Advancement of Science
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  • 1 Laboratory for Biotechnological Research “3D Bioprinting Solutions”, Moscow, Russian Federation
  • 2 A.A. Baikov Institute of Metallurgy and Material Science, Russian Academy of Sciences, Moscow, Russian Federation
  • 3 P.A. Hertsen Moscow Oncology Research Center, National Medical Research Radiological Center, Moscow, Russian Federation
  • 4 Peoples' Friendship University of Russia (RUDN University), Moscow, Russian Federation
  • 5 Riga Technical University, Riga, Latvia
  • 6 Joint Institute for High Temperatures, Russian Academy of Sciences, Moscow, Russian Federation
  • 7 Susquehanna University, Selinsgrove, PA, United States
  • 8 National Research Center “Kurchatov Institute”, Moscow, Russian Federation
  • 9 Imtek Ltd., Moscow, Russian Federation
  • 10 Central Research Institute for Machine Building, Korolev, Moscow Region, Russian Federation
  • 11 Institute of Biomedical Problems, Russian Academy of Sciences, Moscow, Russian Federation
  • 12 Yu.A. Gagarin Research and Test Cosmonaut Training Center, Star City, Moscow Region, Russian Federation
  • 13 Canary Center for Early Cancer Detection, Department of Radiology, Stanford University, Palo Alto, CA, United States
  • 14 Institute for Regenerative Medicine, I.M. Sechenov First Moscow State Medical University, Moscow, 119991, Russian Federation
Magnetism; Predictive analytics; Scaffolds (biology); Space research; Biofabrication; Feasible alternatives; Fusion process; Human chondrocytes; Label-free approach; Space life science; Threedimensional (3-d); Tissue constructs; Tissue; article; computer simulation; human; human tissue; magnetic field; regenerative medicine; tissue engineering
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