Mendeleev Communications.
Royal Society of Chemistry.
Vol. 27.
2017.
P. 116-118
Quasichemical description of the cell death kinetics of cellular biosensor Spirostomum ambigua for testing the biological activity of aqueous solutions
A kinetic method of biotesting of aqueous solutions using isolated cells of unicellular organism Spirostomum ambigua has been described. A kinetic scheme of interaction of cellular biosensor with ligands based on the Michaelis-Menten mechanism was proposed. It was shown that the death rate of S. ambigua featured the Arrhenius-type dependence on temperature. The activation energy was determined for different toxicants and drugs. For many compounds the activation energy of slow stage of the process of cellular biosensor death linearly correlates with parameter DL50 for the same substances at their oral administration to laboratory animals. © 2017, Allerton Press, Inc.
Authors
Goncharuk V.V.
1
,
Syroeshkin A.V.
2
,
Zlatskiy I.A.
1,
2
,
Uspenskaya E.V.
2
,
Orekhova A.V.
2
,
Levitskaya O.V.
2
,
Dobrovolskiy V.I.3
,
Pleteneva T.V.
2
Number of issue
2
Language
English
Pages
97-102
Status
Published
Link
Volume
39
Year
2017
Organizations
- 1 Dumanskii Institute of Colloid and Water Chemistry, National Academy of Sciences of Ukraine, Kiev, Ukraine
- 2 Peoples Friendship University of Russia (RUDN University), Moscow, Russian Federation
- 3 All-Russia Research Institute of Physicotechnical and Radio Engineering Measurements, Moscow, Russian Federation
Keywords
activation energy; biosensor; biotesting of water; kinetic isotope effect; Spirostomum ambigua; toxicity
Date of creation
19.10.2018
Date of change
19.10.2018
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Other records
European Physical Journal C.
Springer New York LLC.
Vol. 77.
2017.