Nonlinear Analysis, Theory, Methods and Applications.
Elsevier Ltd.
Vol. 189.
2019.
Physicochemical Characteristics of a Variant of Chaperon GroEL Apical Domain Designed to Enhance the Expression and Stability of Target Proteins
Abstract: This work describes the properties of a new protein, a modification of GroEL apical domain designed to be a leader in fusion systems. This polypeptide leader demonstrates a high level of expression in a bacterial system; it is soluble and retains its solubility during standard biochemical manipulations. The secondary structure of the protein and its thermostability, as well as the protein solubility, were studied in a wide temperature range. To simplify the subsequent purification of the target protein, the possibility of its chemical cleavage from the fused protein by methionine residues with cyanogen bromide is provided. © 2019, Pleiades Publishing, Inc.
Authors
Kurov K.A.2
,
Savvin O.I.2
,
Yurkova M.S.
2,
1
,
Zenin V.A.
2,
1
,
Nagibina G.S.3
,
Melnik B.S.3
,
Fedorov A.N.
2,
1
Number of issue
8
Language
English
Pages
765-770
Status
Published
Link
Volume
55
Year
2019
Organizations
- 1 People’s Friendship University of Russia, Moscow, 117198, Russian Federation
- 2 Fundamentals of Biotechnology Federal Research Center, Russian Academy of Sciences, Moscow, 119071, Russian Federation
- 3 Institute of Protein Research, Russian Academy of Sciences, Pushchino, Moscow oblast 142290, Russian Federation
Keywords
bacterial expression; chaperon GroEL; fusion system; leader protein
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Other records
Analysis and Mathematical Physics.
Springer Basel.
Vol. 9.
2019.
P. 1991-2019