Impact of the chemical analogues of decarboxylated ornithine and S-adenosylmethionine on the rate of the L-cells growth in the tissue culture

The impact of the aliphatic amino- and oxy derivatives decarboxylated ornithine and S-adenosylmethionine on the rate of the L-cells growth in a tissue culture has been investigated. The known specific inhibitors of the polyamine synthesis enzymes (DFMO and MGBG) were used for control and comparison of efficacy of the substances tested. The nature of action of 1-aminooxy-3-aminopropane (APA) and S-(5'-deoxyadenosile)-S-methyl-β-thioethylhydroxylamine (APA) used separately or combined one with another was similar to the effect of DFMO and MGBG though less expressed. The degree of inhibition of the L-cells growth as of the 4th day made 50-60%. By the 7th day the tendency to not only stabilization of the growth rate but also to the recovery thereof manifested itself. This points to the reversible nature of action of the APA and AMA combinations and constitutes the difference from the effect DFMO and MGBG.