There was investigated the distribution of l/D ACE polymorphism, which predisposes to sports activity among the Arab populations of Algeria and Syria, as well as in Russians from Central Russia. The ACE genotypes were established using polymerase chain reaction (PCR) for 55 healthy Algerians with the mean age of 33.4±11.6 years, 40 Syrians (25.5±7.9 years) and 59 Russians (20.5±6.8 years). A significant difference was found in the ACE genotype distribution between Russians and Algerians (X2 = 40.52; P-0.00019), and between Russians and Syrians (X2 = 19.57; P =0.038). The results show the l/D ACE polymorphism among Arabs and Russians should be considered in the identification of the genetic potential of physical qualities to select the optimal physical activity and develop a personalized approach to training in order to achieve best athletic performance. The research was conducted at the Department of Biology and General Genetics, Medical Institute of Peoples' Friendship University of Russia.