Pharmaceutical Chemistry Journal.
Vol. 52.
2019.
P. 1001-1006
HPLC-MS Method for Simultaneous Quantitative Determination of an Innovative Russian Gestagen and its Metabolites in Rat and Rabbit Blood Sera
An HPLC-MS method for simultaneous quantitative determination of a novel gestagenic pharmaceutical and two of its metabolites in rat and rabbit blood sera was developed and validated. The method was selective and specific. The detection limit for all analytes was 5 ng/mL; lower limit of quantitation, 10 ng/mL. Calibration curves for all analytes had the form y = ax + b. The correlation coefficients were >0.99. Matrix effects, degree of extraction, and stability of analytes in standard solutions, analytical samples, and the biomatrix with multiple freeze—thaw cycles were studied. © 2019, Springer Science+Business Media, LLC, part of Springer Nature.
Authors
Stepanova E.S.
1
,
Makarenkova L.M.
1
,
Chistyakov V.V.
1
,
Rybakov Y.L.2
,
Gukasov V.M.2
,
Fedotcheva T.A.3,
4
,
Parshin V.A.3
,
Votyakov V.A.3
,
Shimanovskii N.L.3
Journal
Number of issue
12
Language
English
Pages
1016-1020
Status
Published
Link
Volume
52
Year
2019
Organizations
- 1 People’s Friendship University of Russia (RUDN University), Moscow, 117198, Russian Federation
- 2 Scientific Research Institute Federal Research Centre for Project Evaluation and Consulting Services, Moscow, 123995, Russian Federation
- 3 N. I. Pirogov Russian National Research Medical University, Ministry of Health of the RF, Moscow, 117997, Russian Federation
- 4 All-Russia Research Institute of Medicinal and Aromatic Plants (VILAR), Russian Academy of Agricultural Sciences, Moscow, 113628, Russian Federation
Keywords
gestagens (progestins); gestobutanoyl; HPLC-MS; validation
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P. 51-70