Identification and expression of haponin, a new protein from HL-60 cells

We found a new protein haponin (an HLDF-like protein) in promyelocyte HL-60 cells that is immunoreactive to polyclonal antibodies against HLDFβ. Determination of the partial primary structure of the protein allowed us to reveal an immunogenic peptide of haponin and, on the basis of the amino acid sequence of this peptide, the degenerate primers were synthesized, which enabled us to clone the full-size cDNA of haponin. The stable heterologous expression of this cDNA in E. coli cells (Rosetta™ strain) was obtained. Preparations of natural and recombinant proteins exhibited antigenic cross-reactivity to polyclonal antibodies against this peptide. © 2007 Pleiades Publishing, Inc.

Authors
Vonarshenko A.V.1 , Radchenko V.V.1 , Gapon M.V.1 , Rodionov I.L.2 , Babichenko I.I. 3 , Kakuev D.L.1 , Artamonov I.D.1 , Garkovenko A.V.1 , Dyachkova L.G.1 , Lipkin V.M.1 , Kostanyan I.A.1
Journal
Russian Journal of Bioorganic Chemistry
Number of issue
6
Language
English
Pages
607-609
Status
Published
Link
External link
DOI
10.1134/S106816200706012X
Volume
33
Year
2007
Organizations
  • 1 Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, Moscow 117997, Russian Federation
  • 2 Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Pushchino Branch, Russian Academy of Sciences, Pushchino, Moscow oblast 142290, Russian Federation
  • 3 Department of Medicine, People's Friendship University of Russia, ul. Miklukho-Maklaya 6, Moscow 117198, Russian Federation
Keywords
Eukaryotic factor of translation initiation eIF1a; Haponin; Human promyelocytic cell line HL-60; STAT1 transcription factor
Date of creation
19.10.2018
Date of change
19.10.2018
Short link
https://repository.rudn.ru/en/records/article/record/3189/
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