Objectives: To compare 3D cephalometric analysis performed using AI with that conducted manually by a specialist orthodontist. Methods: The CBCT scans (a field of view of 15 × 15 cm) used in the study were obtained from 30 consecutive patients, aged 18 to 50. The 3D cephalometric analysis was conducted using two methods. The first method involved manual tracing performed with the Invivo 6 software (Anatomage Inc., Santa Clara, CA, USA). The second method involved using AI for cephalometric measurements as part of an orthodontic report generated by the Diagnocat system (Diagnocat Ltd., San Francisco, CA, USA). Results: A statistically significant difference within one standard deviation of the parameter was found in the following measurements: SNA, SNB, and the left interincisal angle. Statistically significant differences within two standard deviations were noted in the following measurements: the right and left gonial angles, the left upper incisor, and the right lower incisor. No statistically significant differences were observed beyond two standard deviations. Conclusions: AI in the form of Diagnocat proved to be effective in assessing the mandibular growth direction, defining the skeletal class, and estimating the overbite, overjet, and Wits parameter.