Kanamycin and Ofloxacin Activate the Intrinsic Resistance to Multiple Antibiotics in Mycobacterium smegmatis

Drug resistance (DR) in Mycobacterium tuberculosis is the main problem in fighting tuberculosis (TB). This pathogenic bacterium has several types of DR implementation: acquired and intrinsic DR. Recent studies have shown that exposure to various antibiotics activates multiple genes, including genes responsible for intrinsic DR. To date, there is evidence of the acquisition of resistance at concentrations well below the standard MICs. In this study, we aimed to investigate the mechanism of intrinsic drug cross-resistance induction by subinhibitory concentrations of antibiotics. We showed that pretreatment of M. smegmatis with low doses of antibiotics (kanamycin and ofloxacin) induced drug resistance. This effect may be caused by a change in the expression of transcriptional regulators of the mycobacterial resistome, in particular the main transcriptional regulator whiB7.

Authors
Vatlin Aleksey A. 1, 2 , Bekker Olga B.2 , Shur Kirill V.2 , Ilyasov Rustem A.2, 3 , Shatrov Petr A.4 , Maslov Dmitry A.5 , Danilenko Valery N.2
Journal
Biology
Publisher
MDPI
Number of issue
4
Language
English
Pages
506
Status
Published
DOI
10.3390/biology12040506
Volume
12
Year
2023
Organizations
  • 1 Institute of Ecology, Peoples’ Friendship University of Russia (RUDN University), 117198 Moscow, Russia
  • 2 Laboratory of Bacterial Genetics, Vavilov Institute of General Genetics Russian Academy of Sciences, 119333 Moscow, Russia
  • 3 Laboratory of Molecular Genetics, Bashkir State Agrarian University (BSAU), 450001 Ufa, Russia
  • 4 Phystech School of Biological and Medical Physics, Moscow Institute of Physics and Technology (National Research University), 141701 Dolgoprudny, Russia
  • 5 Division of Gastroenterology and Hepatology, Department of Medicine, Stanford University School of Medicine, Stanford, CA 94305, USA
Date of creation
14.11.2024
Date of change
14.11.2024
Short link
https://repository.rudn.ru/en/records/article/record/157734/
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