VERIFICATION OF EPIDEMIOLOGICAL TRENDS IN ALLERGY USING IMMUNE MARKERS

Using standardized methods and methods of phased diagnostics for allergic diseases - is considered as important precondition for final verification of diagnosis. Consequently, it would be reasonable to identify immunologic sta- tus in all children with allergic diseases. The above-mentioned defined the purpose of the study: seeking for differ- entiated markers among the generally recognized immunological laboratory parameters in children with both diag- nosed and primary-diagnosed allergic diseases. 243 children, 7-8 and 13-14 of ages, were interviewed and subse- quently examined (part of the children) by random selection method, in western Georgia. Diagnosis included two phases of examination: I. Phase - questionnaire screening using international questionnaire ISAAC (International Study of Asthma and Allergies in Childhood); II. Phase - immune-laboratory examination - detection of blood immunological indices by immunofluorescence method. Questionnaire screening allowed distinguishing: I group of children with primary-diagnosis of allergic diseases (37). II group of children with basic diagnosis of allergic diseases (19). III group of the children - practically healthy ones (20), who didn’t reveal any anomalies in accord- ance with the international questionnaire ISAAC. Through the Questionnaire screening 56 children (23 %) with positive tests were selected. In group I as well as in group II in comparison with group III were revealed: increased total IgE index -204,3±67.0, 239±87,6 МЕ/ml (p<0.05 )respectively, reduced content of T- lymphocytes (CD3) - 39.44±5.437; 40.2±1.01МЕ/ml (p<0.05 ), respectively; T-helpers (CD4) 23.66±0.897; 26±0.124 (p<0.05) and ac- tivated cytotoxic T- lymphocytes (CD45RA) 38.6±0.581; 39.87±0.870 (p<0.05). Revealing the changes in immu- nologic indexes with appropriate clinical evidences might serve as precondition and base for conducting appropri- ate therapy in given groups of children with both diagnosed and non-diagnosed allergic diseases.