OXIDOREDUCTASE AND LECTIN SYSTEMS REACTING TO GLYCOCONJUGATES IN BIOPREPARATIONS OF MEDICAL ORIENTATION ACCORDING TO CHEMILUMINESCENCE OF A LIVE IMAGE

Screening of oxidoreductases of probiotic and plant origin in preparations of preventive and medical importance was carried out. Systems of weakly acidic lactobacilli oxidoreductases (pI 5-5.6) and strongly acidic plant oxidoreductases (pI 3.2-3.7) were identified and registered. Oxidoreductases showed sensitivity to glycoconjugates and could coincide with the location of lectins. Highly sensitive methods of identification, purification, separation and strong immobilization on membranes of oxidoreductase systems using isoelectrofocusing in gel, electroblotting, chemiluminescence of substrate oxidation products in the mode of registration of stepwise kinetics of luminescence are proposed. Methods of identification and registration of both oxidoreductases and lectins are promising for standardization of biological products, assessment of the presence of glycoconjugates in them, and the use of drugs as biosensors. In an isolated, immobilized form, oxidoreductase systems cofunctioning to lectins and glycoconjugates are promising for use in microanalysis, including as biosensors of any exposed (available) glycoconjugates.