Bulletin of the Russian Academy of Sciences: Physics. Vol. 84. 2020. P.. 1390-1393
Preparative Purification of Pseudomonas aeruginosa Bacteriophages via the Combination of Gel-Permeation and Anion-Exchage Chromatography
Abstract: We developed and described a method for the purification of Pseudomonas aeruginosa bacteriophages that consists of sequential steps of size-exclusion and anion-exchange chromatography. The technique was tested on five bacteriophages of different size and morphology. Similar results were obtained, which allows the proposed approach to be considered universal and highly scalable. © 2020, Pleiades Publishing, Inc.
Authors
Landyshev N.N. 1 , Voronko Y.G. 1 , Kulikov E.E. 2 , Sykilinda N.N. 3 , Miroshnikov K.A. 3
Issue number
6
Language
English
Pages
685-691
State
Published
Link
Volume
56
Year
2020
Organizations
- 1 Institute of Medicine, Peoples’ Friendship University (RUDN University), Moscow, 117198, Russian Federation
- 2 Winogradsky Institute of Microbiology, Federal Research Center Fundamentals of Biotechnology, Russian Academy of Sciences, Moscow, 117312, Russian Federation
- 3 Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, 117997, Russian Federation
Keywords
anion-exchange chromatography; bacteriophages; controlled-pore glass; Pseudomonas aeruginosa
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