PROBLEMS OF YEAR-ROUND MICROPROPIATION OF STRAWBERRY AND NEW APPROACHES TO THEIR SOLUTION

Clonal micropropagation of strawberries can significantly accelerate the start of mass use of new highly productive cultivars. Mostly such material is needed for planting nurseries. However, the discrepancy between the annual working rhythm of the laboratory and the culture development cycle in natural conditions requires solving the problems of ensuring the viability of microplants during the period from late August to early March before planting in the greenhouse. At this time, it is often impossible to provide adequate conditions for the adaptation of microplants due to the low influx of sunlight and the high cost of using artificial sources. Purpose. The purpose of the study was to develop a new scheme for working with test tube material obtained in the autumn-winter-spring period, which would eliminate the need for its adaptation immediately after the rooting of microcuttings. Materials and methods. Strawberry cultivars of foreign selection Kimberly, Asia and Florence are studied. Cultivation was carried out on Murashige-Skoog medium at a temperature of 23±1°C, 16-hour photoperiod, illumination of 5-6 kLx. In microplants obtained within 1.5-2 months, after rooting, the formed roots and leaves were removed. Next, they were transplanted in the form of vegetating buds onto fresh medium containing auxin at a concentration of 0.1 mg L-1 for rehabilitation. Planting was carried out with 6-7 vegetating buds in 250 ml flasks. Results. The restoration of leaves and roots of vegetating buds took place within 20-30 days, and their safety and survival rate was 100%. Subsequent repetitions of this procedure were also successful. Conclusion. The new technique allows you to accurately plan the release of adapted strawberry microplants throughout the year. The material accumulated in the laboratory can be immediately adapted in a greenhouse with sufficient sunlight. © 2024, Science and Innovation Center Publishing House. All rights reserved.