Osteoinductive Moldable and Curable Bone Substitutes Based on Collagen, BMP-2 and Highly Porous Polylactide Granules, or a Mix of HAP/β-TCP

In dentistry, maxillofacial surgery, traumatology, and orthopedics, there is a need to use osteoplastic materials that have not only osteoinductive and osteoconductive properties but are also convenient for use. In the study, compositions based on collagen hydrogel were developed. Polylactide granules (PLA) or a traditional bone graft, a mixture of hydroxyapatite and β-tricalcium phosphate (HAP/β-TCP), were used for gel filling to improve mechanical osteoconductive properties of compositions. The mechanical tests showed that collagen hydrogels filled with 12 wt% highly porous PLA granules (elastic modulus 373 ± 55 kPa) or 35 wt% HAP/β-TCP granules (elastic modulus 451 ± 32 kPa) had optimal manipulative properties. All composite components were cytocompatible. The cell’s viability was above 90%, and the components’ structure facilitated the cell’s surface adhesion. The bone morphogenetic protein-2 (BMP-2) provided osteoinductive composition properties. It was impregnated directly into the collagen hydrogel with the addition of fibronectin or inside porous PLA granules. The implantation of a collagen hydrogel with BMP-2 and PLA granules into a critical-size calvarial defect in rats led to the formation of the most significant volume of bone tissue: 61 ± 15%. It was almost 2.5 times more than in the groups where a collagen-fibronectin hydrogel with a mixture of HAP/β-TCP (25 ± 7%) or a fibronectin-free composition with porous PLA granules impregnated with BMP-2 (23 ± 8%) were used. Subcutaneous implantation of the compositions also showed their high biocompatibility and osteogenic potential in the absence of a bone environment. Thus, the collagen-fibronectin hydrogel with BMP-2 and PLA granules has optimal biocompatibility, osteogenic, and manipulative properties.

Vasilyev Andrey Vyacheslavovich 1, 2, 6, 7 , Kuznetsova Valeriya Sergeevna 1, 2 , Bukharova Tatyana Borisovna 1 , Osidak Egor Olegovich3 , Grigoriev Timofei Evgenevich 5, 8 , Zagoskin Yuriy Dmitrievich 5 , Nedorubova Irina Alekseevna1 , Domogatsky Sergey Petrovich3, 4 , Babichenko Igor Ivanovich 2, 6 , Zorina Oksana Aleksandrovna2, 7 , Kutsev Sergey Ivanovich1 , Chvalun Sergei Nicolaevich 5 , Kulakov Anatoly Alekseevich 2 , Losev Fedor Fedorovich2, 7 , Goldshtein Dmitry Vadimovich 1
Номер выпуска
  • 1 Research Centre for Medical Genetics
  • 2 Central Research Institute of Dental and Maxillofacial Surgery
  • 3 Imtek Ltd.
  • 4 Federal State Budgetary Institution National Medical Research Center of Cardiology Ministry of Health of the Russian Federation
  • 5 NRC “Kurchatov Institute”
  • 6 Российский университет дружбы народов
  • 7 I.M. Sechenov First Moscow State Medical University of the Ministry of Health of the Russian Federation (Sechenov University)
  • 8 Moscow Institute of Physics and Technology (National Research University)
Ключевые слова
collagen; fibronectin; hydrogel; polylactide; HAP/β-TCP; BMP-2; osteoinduction
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Другие записи

Vasilyev Andrey Vyacheslavovich, Kuznetsova Valeriya Sergeevna, Bukharova Tatyana Borisovna, Grigoriev Timofei Evgenevich, Zagoskin Yuriy Dmitrievich, Nedorubova Irina Alekseevna, Babichenko Igor Ivanovich, Chvalun Sergey Nicolaevich, Goldstein Dmitry Vadimovich, Kulakov Anatoliy Alekseevich
Molecules. MDPI AG. Том 26. 2021. 261 с.