The forensic implications of the relationship between the proteolytic enzymes activity and the changes in nadh and fad fluorescence intensity in skeletal muscle when determining the time of death (Experimental study)

Objective — evaluation of the relationship between the activity of proteolytic enzymes (cathepsin D and calpains) and the dynamics of the fluorescence intensity of the coenzymes nicotinamide adenine dinucleotide (NADH) and flavin adenine dinucleotide (FAD) in the rats’ skeletal muscles in relation to the time of death. The proteolytic activity of enzymes in rat skeletal muscle was determined at the postmortem time points corresponding to the most significant changes in the dynamics of coenzymes NADH and FAD fluorescence intensity. The proteolytic enzymes activity was found to be low during the period of increasing intensity of NADH fluorescence observed within 3 hours after death. An increase in the activity of proteolytic enzymes was registered in 4.5 hours after death which corresponds to the initial point of decrease in NADH fluorescence intensity. In 24 hours post-mortem, corresponding to increased FAD fluorescence intensity a significant decrease in the activity of calpains was found. The results of the study suggest that the nature of the postmortem dynamics of the fluorescence intensity of coenzymes is largely due to the peculiarities of intracellular proteolysis. The study results suggest that the pattern of post mortem changes in coenzyme fluorescence intensity is largely attributable to the specifics of intracellular proteolysis. The relationship between coenzyme fluorescence and molecular mechanisms of cell death confirms the viability and feasibility of laser-induced spectroscopy for post-mortem changes assessment when determining the time of death. © 2021, Media Sphera Publishing Group. All rights reserved.

Номер выпуска
  • 1 Medical Institute of the Russian Peoples’ Friendship University, Moscow, Russian Federation
  • 2 V.A. Negovsky Research Institute of General Reanimatology, Federal Scientific and Clinical Center of Reanimatology and Rehabilitation, Moscow, Russian Federation
Ключевые слова
Autolysis; Calpains; Cathepsin D; NADH; Time of death determination
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Akoulina E.A., Demianova I.V., Zharkova I.I., Voinova V.V., Zhuikov V.A., Khaydapova D.D., Chesnokova D.V., Menshikh K.A., Dudun A.A., Makhina T.K., Bonartseva G.A., Volkov A.V., Asfarov T.F., Ivanov S.Y., Shaitan K.V., Bonartsev A.P.
Бюллетень экспериментальной биологии и медицины Клеточные технологии в биологии и медицине. New York Consultants BureauSpringer / Автономная некоммерческая организация Издательство Российской академии медицинских наук. Том 171. 2021. С. 172-177