Recombinant IgA1 protease from N. meningitidis. obtaining and properties

On the base of nucleotide sequence, coding IgA1 protease from Neisseria meningitidis serogroup B, strain ÌÑ58, which was determined from data base http://www.ncbi.nlm.nih.gov/Genbank), recombinant plasmide DNA was created comprising nucleotide sequence of IgA1 protease, strain H44/76, providing IgA1 protease expression in the host-cell (pBIGAPS1). The method of expression, isolation, purification and refolding of recombinant enzyme was developed. IgA1 protease exhibits high specificity and cleaves only IgA1, but not IgG. Immunogenic and protective properties of IgA1 protease to meningococcus of serogroup À, Â and Ñ were shown. Obtained enzyme can be considered as perspective polyvaccine candidate for prophylaxis against meningococcus infection, induced by bacteria N. meningitidis and especially against serogroup B.

Авторы
Serova O.V.1 , Melnikov E.E.1 , Zinchenko A.A.1 , Kotel'nikova O.V. 1 , Alliluev A.P. 3 , Bichucher A.M.2 , Gordeeva E.A.1 , Zhigis L.S.1 , Zueva V.S.1 , Kozlov L.V.2 , Melikhova T.D.1 , Nokel E.A.1 , Yagudaeva E.Y.1 , Rumsh L.D.1
Редакторы
-
Издательство
-
Номер выпуска
6
Язык
Английский
Страницы
42-47
Статус
Опубликовано
Подразделение
-
DOI
-
Номер
-
Том
3
Год
2011
Организации
  • 1 Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya, 16/10, 117997 Moscow, Russian Federation
  • 2 FGUN Gabrichevskii Moscow Research Institute of Epidemiology and Microbiology, ul. Admirala Makarova, 10, 125212 Moscow, Russian Federation
  • 3 Medical Faculty, Russian University of Peoples' Friendship, ul. Miklukho-Maklay, 6, 117198, Moscow, Russian Federation
Ключевые слова
Enzymatic activity; IgA1 protease; Immunogenicity; Isolation; Meningococcus
Дата создания
19.10.2018
Дата изменения
19.10.2018
Постоянная ссылка
https://repository.rudn.ru/ru/records/article/record/2454/