Consumption of triazine herbicide atrazine by laccase-positive and laccase-negative strains of soil fungus Mycelia sterilia INBI 2-26

Asporogenic fungus Mycelia sterilia INBI 2-26 isolated from tropical soils with high residual dioxin content (as a result of Agent Orange defoliant treatment during the Vietnamese-American war) and capable of atrazine decomposition was treated to obtain protoplasts. This technique resulted in isolation of laccase-positive and laccase-negative clones. Atrazine consumption by liquid surface cultures of Mycelia sterilia INBI 2-26 was monitored by using enzyme immune assay and reversed-phase HPLC. Atrazine (20 μg/ml) stimulated fungal growth. The laccase-positive clone consumed up to 80% of atrazine within four weeks. However, no correlation of atrazine consumption and laccase activity in the culture medium was observed. Moreover, the laccase-negative clone was also capable of consuming at least 60-70% of atrazine within three weeks. Surprisingly, in the corresponding control set (cultivation of laccase-negative clone without atrazine) an unidentified metabolite having a retention time and UV-spectrum similar to those of atrazine was also found. It was concluded that the presence of laccase was not a crucial factor in atrazine consumption by this fungus.

Vasil'chenko L.G.1 , Khromonygina V.V. 1, 2 , Koroleva O.V.1 , Landesman E.O.1 , Gaponenko V.V.3 , Kovaleva T.A.3 , Kozlov Yu.P. 2 , Rabinovich M.L. 1, 2
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  • 1 Bach Institute of Biochemistry, Russian Academy of Sciences, Leninskii pr. 33, Moscow, 119071, Russian Federation
  • 2 Faculty of Ecology, Russ. Univ. of People's Friendship, Moscow, 113093, Russian Federation
  • 3 Inst. Rsrc. Savings Ecol./Serv. E., Moscow State University of Service, Moscow oblast, 141221, Russian Federation
Ключевые слова
2,4,5 trichlorophenoxyacetic acid; atrazine; defoliant agent; dioxin; herbicide; laccase; triazine derivative; article; biological monitoring; clone; controlled study; correlation analysis; culture medium; decomposition; enzyme activity; enzyme linked immunosorbent assay; fungal strain; fungus culture; fungus growth; fungus isolation; liquid; metabolite; mycelia sterilia; mycelium; nonhuman; protoplast; reversed phase high performance liquid chromatography; soil; stimulation; technique; tropics; ultraviolet radiation; war; Fungi
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