In this work, the study of the effectiveness of the experimental biopreparation Likvidator 2 on the bio-organic waste of fish farms and on the chemical composition of compounds released during the biodegradation of waste was carried out. Samples of volatile organic compounds (VOC) released during the decomposition of fish waste from OOO VICTAN trout farm were taken using the method of sample aspiration on sorption tubes and liquid extraction; a method of thermal desorption of VOC samples adsorbed on a layer of a stationary carrier was developed and applied; the composition of VOC desorption products and extracts was studied by gas-chromatography-mass spectrometry with electron ionization. The regularities of changes in the composition of VOC, emitted during biodegradation of fish waste, including the addition of the experimental biopreparation and the effectiveness of its use were assessed.