Amyloid-beta induced cell death is independent of free radicals

Acutely dissociated rat cerebellar granule cell neurons were incubated with amyloid-beta (1-42) and studied by flow cytometry. Amyloid-beta caused a dose-dependent loss of viability, as determined by intracellular accumulation of propidium iodide (PI),and that was not accompanied by significant elevation of intracellular calcium, measured by Fluo-3 or reactive oxygen species (ROS), measured by 2,7'-dihydro-dichlorfluorescein diacetate (DCF). Carnosine, a ROS scavenger and an inhibitor of non-enzymatic glycosylation, partially reduced cell death caused by amyloid-beta. We conclude that amyloid-beta causes a relatively acute loss of cell viability in cerebellar granule cell neurons, which does not result from either elevation of intracellular calcium concentration or generation of ROS.

Authors
Boldyrev A. , Koudinov A. , Berezov T. , Carpenter D.O.
Editors
-
Publisher
IOS Press
Number of issue
6
Language
English
Pages
633-638
Status
Published
Department
-
Link
-
DOI
-
Number
-
Volume
6
Year
2004
Organizations
-
Keywords
flow cytometry; reactive oxygen species; calcium; carnosine; cerebellum; granule cells; Alzheimer's disease
Date of creation
19.10.2018
Date of change
19.10.2018
Short link
https://repository.rudn.ru/en/records/article/record/8872/