SINGLE-MODE PROPAGATION OF ADIABATIC GUIDED MODE IN A SMOOTH TRANSITION BETWEEN PLANAR WAVEGUIDES
Article
Progress in Biomedical Optics and Imaging - Proceedings of SPIE.
SPIE.
Vol. 12193.
2022.
Time-resolved emission spectroscopy to elucidate the functional nature of heat-stable transcription factor
A 22 kDa protein from Thermus thermophilus is characterised as a DNA binding transcription regulator and its function is established using the fluorescence spectroscopy technique. The steady-state fluorescence spectroscopy result shows significant binding of calf thymus DNA and protein molecule. To confirm, the DNA quenching effect in real-time, a time-resolved emission spectroscopy study was performed and the result shows good agreement with steady-state quenching analysis. © 2022 Wiley Periodicals LLC.
Authors
Kutumbarao N.V.1,
2
,
Karthikeyan S.
3,
4,
5
,
Ganesan S.3
,
Velmurugan D.1,
6
Journal
Language
English
Status
Published
Link
Year
2022
Organizations
- 1 Centre of Advanced Study in Crystallography and Biophysics, University of Madras, Tamil Nadu, Chennai, India
- 2 Institute of Transformative Bio-Molecules (ITbM), Nagoya University, Nagoya, Japan
- 3 Department of Medical Physics, Anna University, Tamil Nadu, Chennai, India
- 4 Department of Organic Chemistry, Science Faculty, Peoples’ Friendship University of Russia (RUDN University), Moscow, Russian Federation
- 5 School of Advanced Sciences, Physics Division, Vellore Institute of Technology University, Chennai Campus, Tamil Nadu, Chennai, India
- 6 Department of Biotechnology, School of Bioengineering, SRM Institute of Science and Technology, Tamil Nadu, Kattankulathur, India
Keywords
calf thymus (ct) DNA; purification; Thermus thermophilus; time correlated single photon counting
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Other records
Lecture Notes in Electrical Engineering.
Springer Verlag.
Vol. 827 LNEE.
2022.
P. 839-845