The study aims to evaluate the effectiveness of standard and modified settings of the spectral color allocation technology in the differential diagnosis of the colon morphological type of epithelial formations. Materials and methods: video colonoscopy using standard and modified mode of technology. A total of 746 patients were selected for spectral colour selection, of whom 80 were selected. There were 36 men, 44 women. The average age of the patients was 60 ± 9 years. There were 171 epithelial fomations, of which 106 (61.9%) were located in the left side of the large intestine: in the rectum - 33 (31.2%), in sigmoid - 52 (49%), in descending part - 21 (19.8%). In the right parts of the colon there were significant lower number of formations - 65 (38.1%): ascending intestine - 26 (40%), transversely - colon - 32 (49.2%), cecum - 7 (10.8%). Endoscopic studies were performed according to standard method using video colonoscopes from Fujinon, EC-590ZW / L, EC-530WL. Results: statistically revealed that the probability of determining the correct morphological type of epithelium is formation is 6 times higher when using the modified spectral color technology highlight compared to standard technology settings. Conclusion: the modified settings of the technology of spectral color highlighting allow much more accurative performance an optical biopsy of epithelial colon formations. © 2020 Global Media Tekhnologii. All rights reserved.