Evaluation of tissue metal and trace element content in a rat model of non-alcoholic fatty liver disease using ICP-DRC-MS

The primary objective of the study was to assess the level of metals and trace elements in liver, serum, and hair of rats with diet-induced non-alcoholic fatty liver disease (NAFLD) using inductively coupled plasma dynamic reaction cell mass spectrometer (ICP-DRC-MS). 56 female 3-months-old Wistar rats divided into two equal groups were fed either standard (10% calories from fat) or high-fat high-carbohydrate diet (60% calories from fat in chow and 10% sucrose solution) for 6 weeks. Serum was examined for insulin resistance markers, lipid profile, and alanine aminotransferase (ALT) activity. Liver histology was assessed after hematoxylin and eosin staining. Metal and trace element concentrations were assessed by means of ICP-DRC-MS. Overfed animals were characterized by higher values of morphometric parameters. Liver examination revealed large and small droplet steatosis, hepatocyte ballooning and necrosis, being characteristic for NAFLD. Animals with NAFLD were characterized by insulin resistance, atherogenic changes of lipid profile and increased ALT activity. Significantly decreased hepatic Co, Cu, I, Li, Mn, Se, Zn levels were observed in rats with NAFLD. At the same time, only hepatic Mn and Se levels remained decreased after adjustment for total protein. Overfed animals were characterized by significantly lower I, Li, and Mn levels in blood serum, whereas concentration of Co, Se, V, and Sr exceeded the control values. In general, the results of the study demonstrate that NAFLD significantly affects metal and trace element status in experimental animals. © 2016 Elsevier GmbH

Authors
Gatiatulina E.R.1 , Popova E.V.1 , Polyakova V.S.2 , Skalnaya A.A.3 , Agletdinov E.F.4 , Nikonorov A.A.1, 5 , Skalny A.V. 5, 6, 7, 8 , Tinkov A.A. 1, 5, 6, 8
Publisher
Elsevier GmbH
Language
English
Pages
91-99
Status
Published
Volume
39
Year
2017
Organizations
  • 1 Department of Biochemistry, Orenburg State Medical University, Sovetskaya St., 6Orenburg 460000, Russian Federation
  • 2 Department of Pathologic Anatomy, Orenburg State Medical University, Sovetskaya St., 6Orenburg 460000, Russian Federation
  • 3 Faculty of Fundamental Medicine, Lomonosov Moscow State University, Lomonosovsky Prospekt, 31-5, Moscow, 117192, Russian Federation
  • 4 Central Research Laboratory, Bashkir State Medical University, Zaki Validi St., 64/2, Ufa, 450057, Russian Federation
  • 5 Institute of Bioelementology (Russian Satellite Centre of Trace Element − Institute for UNESCO), Orenburg State University, Pobedy Ave. 13Orenburg 460352, Russian Federation
  • 6 Laboratory of biotechnology and applied bioelementology, Yaroslavl State University, Sovetskaya st., 14, Yaroslavl, 150000, Russian Federation
  • 7 All-Russian Research Institute of Medicinal and Aromatic Plants (VILAR), Grina St., 7, Moscow, 117216, Russian Federation
  • 8 RUDN University, Miklukho-Maklai str. 6, Moscow, 117198, Russian Federation
Keywords
Fatty liver; Insulin resistance; Minerals; Obesity; Trace elements
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