Microcapsules of poly(3-hydroxybutyrate) for sustained protein release

The aim of investigation was to develop a system for protein sustained release based on the use of poly(3-hydroxybutyrate) (PHB) microcapsules loaded with bovine serum albumin (BSA). Materials and Methods. To develop microcapsule we used PHB obtained microbiologically by a strain-producer Azotobacter chroococcum 7B. Microcapsules loaded with model protein BSA were produced by double emulsion technique.“water/oil/water” Morphology of microcapsules was investigated by methods of confocal and scanning electron microscopy, when the loading and release of BSA was examined spectrophotometrically. In vivo biocompatibility of microcapsules was studied in accordance with intramuscular implantation and histology findings. Results. The study of BSA incorporation and its sustained release from microcapsules for more than 190 h demonstrated the efficacy of proposed system. The mechanism of protein release was found to occur due to the rupture of polymer walls. Moderate tissue response to the implantation of obtained microcapsules was demonstrated. Conclusion. Developed PHB microcapsules loaded with BSA are good model of long acting protein drugs. © 2015, Nizhny Novgorod State Medical Academy. All rights reserved.

Authors
Zernov A.L.1, 6 , Ivanov E.A.1 , Makhina T.K.1 , Myshkina V.L.1 , Samsonova O.V.3 , Feofanov A.V.3 , Volkov A.V. 4 , Gazhva J.V.2 , Muraev A.A. 5 , Ryabova V.M.2 , Shaitan K.V.3 , Ivanov S.Y. 2, 5 , Bonartseva G.A.1 , Bonartsev A.P.6, 3, 7
Publisher
Nizhny Novgorod State Medical Academy
Number of issue
4
Language
English
Pages
50-56
Status
Published
Volume
7
Year
2015
Organizations
  • 1 Laboratory of Microorganism Nitrogen Fixation, A.N. Bach Institute of Biochemistry Federal Research Center ‘Fundamentals of Biotechnology’, Russian Academy of Sciences, 33, bld. 2 Leninsky Prospect, Moscow, 119071, Russian Federation
  • 2 Department of Maxillaofacial Surgery and Implantology, Nizhny Novgorod State Medical Academy, 10/1 Minin and Pozharsky Square, Nizhny Novgorod, 603005, Russian Federation
  • 3 Department of Bioengineering, Lomonosov Moscow State University, GSP-1 Leninskie Gory, Moscow, 119991, Russian Federation
  • 4 Department of General Pathology, Central Research Institute of Dental and Maxillofacial Surgery, Ministry of Health of the Russian Federation, 16 Timura Frunze St, Moscow, 119991, Russian Federation
  • 5 Department of Maxillofacial Surgery and Surgical Dentistry, Peoples Friendship University of Russia, 6 Miklukho-Maklaya St, Moscow, 117198, Russian Federation
  • 6 Nizhny Novgorod State Medical Academy, 10/1 Minin and Pozharsky Square, Nizhny Novgorod, 603005, Russian Federation
  • 7 A.N. Bach Institute of Biochemistry Federal Research Center ‘Fundamentals of Biotechnology’, Russian Academy of Sciences, 33, bld. 2 Leninsky Prospect, Moscow, 119071, Russian Federation
Keywords
Biocompatibility; Biodegradable polymers; Microencapsulation; Poly(3-hydroxybutyrate); Polyhydroxyalkanoates; Prolonged release
Date of creation
19.10.2018
Date of change
27.08.2021
Short link
https://repository.rudn.ru/en/records/article/record/4805/
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