EXPERIMENTAL MODEL FOR ASSESSING THE EFFECTIVENESS OF RADIOPROTECTORS IN BIOMEDICAL RESEARCH; [ЭКСПЕРИМЕНТАЛЬНАЯ МОДЕЛЬ ДЛЯ ОЦЕНКИ ЭФФЕКТИВНОСТИ РАДИОПРОТЕКТОРОВ В БИОМЕДИЦИНСКИХ ИССЛЕДОВАНИЯХ]

Background. Radiobiological research on chemical radioprotectors is relevant in the study of radiation injuries and in practical medicine during radiation therapy ofcancer patients. Selection ofthe optimalexperimentalmodelis necessary to assess the effect of radioprotectors on hematopoiesis and is an important task. The aim. Analysis of the possibility of using Danio rerio fish as a test object for assessing the effectiveness of the protective effect of radioprotectors against ionizing radiation using an erythrocyte micronucleus test. Material and methods. The work was carried out on Danio rerio aquarium fish. Seven groups of two-month-old zebrafish juveniles (n = 35), as well as adult fish, were exposedto X-ray radiation atdoses of 0Gy (control), 1.0Gy, 2.0Gy, 3.0Gy, 4.0Gy, 6.0 Gy, 8.0 Gy to determine the frequency of occurrence of micronuclei in peripheral blood erythrocytes 72 hours after irradiation. Results. The frequency of occurrence of micronuclei in Danio rerio erythrocytes was dose-dependent. In juveniles, as in adult fish, the dose of ionizing radiation increased to 4 Gy led to a sharp increase in the frequency of occurrence of micronuclei inerythrocytes. Adult Danio rerio were more radio resistant; the number of micronuclei formedin erythrocytes in two-month-old juveniles exceeded the frequency of occurrence of micronuclei in adult fish. Conclusion. Amodelusing juvenile fishDanio rerio as a laboratory testobjectfor assessing the effectiveness of the protective effect of radioprotectors against ionizing radiation using the erythrocyte micronucleus test can be recommended for testing the effect of medications in case ofradiation injuries, as wellas for assessing the possibility of their use in radiation therapy in cancer patients. © 2024 Scientific Centre for Family Health and Human Reproduction Problems. All rights reserved.